Tissue Culture

Plant cell and tissue transformation is the process through which the endogenous DNA is modified to understand or enhance the plants genetic makeup. Using tissue culture, new plant or cell lines are generated and from these transformed events which host the modified DNA within their genomes.

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Culture Growth Space

 

The facility contains about 812 sq. ft. of high quality culture growth space comprised of three large computer-controlled walk-in tissue culture rooms adding about 540 square feet of growth space (shelf and shaker), ten Percival Scientific CU36L5 chamber units adding an additional 272 sq. ft. of culture space, and about 144 sq. ft. of lighted shelving units. The three large culture rooms are computer- controlled to regulate photoperiod, temperature, and light intensity allowing researchers to place their cultures in near optimal conditions. Culture flexibility is gained by having ten Percival chamber units allowing researchers to set specific temperature and photoperiod controls needed for smaller scale experiments that need more precise light and temperature parameters not offered in the large culture rooms. Having the 144 sq ft of lighted shelf space allows for a place where cultures can acclimate before moving to the greenhouse.

Facility Equipment

 

The facility contains almost all the essential equipment necessary for plant cell and tissue culture and transformation.

  • Fourteen laminar flow tissue culture hoods
  • Refrigerated Eppendorf centrifuge
  • Bench top Eppendorf microfuge
  • Shimadzu spectrophotometer
  • Seven Olympus dissecting microscopes (one with photo capabilities)
  • Inverted microscope
  • Incubators (both wet and dry)
  • Culture incubator/shaker
  • Two floor model temperature controlled shakers
  • BioRad GenePulser electroporator
  • BioRad PDS-1000 gene delivery system
  • A hand-held BioRad Helios system