Jan Jaworski

Dr. Jaworski is a plant biochemist who has been conducting research on plant oils and seed biology since 1972. In recent years, his lab has been using molecular tools to modify oil seeds to better understand how scientists can rationally engineer oil seeds for more oil for biofuels and industrial applications. He graduated from College of the Holy Cross in 1968 and received his Ph. D. from Purdue University in 1972. After 28 years on the faculty in the Chemistry and Biochemistry Department of Miami University in Ohio, he moved to the Danforth Center in 2002.

RESEARCH | PUBLICATIONS | RESEARCH TEAM | LAB NEWS

Publications

Han, J., Clement, J.M., Li, J., King, A., Ng, S., and Jaworski, J.G. (2010). The cytochrome P450 CYP86A22 is a fatty acyl-CoA omega-hydroxylase essential for Estolide synthesis in the stigma of Petunia hybrida. J Biol Chem 285, 3986-3996
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Chen, M., Markham, J.E., Dietrich, C.R., Jaworski, J.G., and Cahoon, E.B. (2008). Sphingolipid long-chain base hydroxylation is important for growth and regulation of sphingolipid content and composition in Arabidopsis. Plant Cell 20, 1862-1878
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King, A., Nam, J.W., Han, J., Hilliard, J., and Jaworski, J.G. (2007). Cuticular wax biosynthesis in petunia petals: cloning and characterization of an alcohol-acyltransferase that synthesizes wax-esters. Planta 226, 381-394
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Markham, J.E., and Jaworski, J.G. (2007). Rapid measurement of sphingolipids from Arabidopsis thaliana by reversed-phase high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. Rapid Commun Mass Spectrom 21, 1304-1314
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Tsegaye, Y., Richardson, C.G., Bravo, J.E., Mulcahy, B.J., Lynch, D.V., Markham, J.E., Jaworski, J.G., Chen, M., Cahoon, E.B., and Dunn, T.M. (2007). Arabidopsis mutants lacking long chain base phosphate lyase are fumonisin-sensitive and accumulate trihydroxy-18:1 long chain base phosphate. J Biol Chem 282, 28195-28206
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Blacklock, B.J., and Jaworski, J.G. (2006). Substrate specificity of Arabidopsis 3-ketoacyl-CoA synthases. Biochem Biophys Res Commun 346, 583-590
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Markham, J.E., Li, J., Cahoon, E.B., and Jaworski, J.G. (2006). Separation and identification of major plant sphingolipid classes from leaves. J Biol Chem 281, 22684-22694
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Paul, S., Gable, K., Beaudoin, F., Cahoon, E., Jaworski, J., Napier, J.A., and Dunn, T.M. (2006). Members of the Arabidopsis FAE1-like 3-ketoacyl-CoA synthase gene family substitute for the Elop proteins of Saccharomyces cerevisiae. J Biol Chem 281, 9018-9029
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Zhang, Y., Li, S.Z., Li, J., Pan, X., Cahoon, R.E., Jaworski, J.G., Wang, X., Jez, J.M., Chen, F., and Yu, O. (2006). Using unnatural protein fusions to engineer resveratrol biosynthesis in yeast and Mammalian cells. J Am Chem Soc 128, 13030-13031
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Jaworski, J., and Cahoon, E.B. (2003). Industrial oils from transgenic plants. Curr Opin Plant Biol 6, 178-184
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Blacklock, B.J., and Jaworski, J.G. (2002). Studies into factors contributing to substrate specificity of membrane-bound 3-ketoacyl-CoA synthases. Eur J Biochem 269, 4789-4798
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Ghanevati, M., and Jaworski, J.G. (2002). Engineering and mechanistic studies of the Arabidopsis FAE1 b-ketoacyl-CoA synthase, FAE1 KCS. Eur J Biochem 269, 3531-3539
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Dehesh, K., Tai, H., Edwards, P., Byrne, J., and Jaworski, J.G. (2001). Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis. Plant Physiol 125, 1103-1114
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Ghanevati, M., and Jaworski, J.G. (2001). Active-site residues of a plant membrane-bound fatty acid elongase beta-ketoacyl-CoA synthase, FAE1 KCS. Biochim Biophys Acta 1530, 77-85
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White, J.A., Todd, J., Newman, T., Focks, N., Girke, T., de Ilarduya, O.M., Jaworski, J.G., Ohlrogge, J.B., and Benning, C. (2000). A new set of Arabidopsis expressed sequence tags from developing seeds. The metabolic pathway from carbohydrates to seed oil. Plant Physiol 124, 1582-1594
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Todd, J., Post-Beittenmiller, D., and Jaworski, J.G. (1999). KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana. Plant J 17, 119-130
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Hlousek-Radojcic, A., Evenson, K.J., Jaworski, J.G., and Post-Beittenmiller, D. (1998). Fatty acid elongation is independent of acyl-coenzyme A synthetase activities in leek and Brassica napus. Plant Physiol 116, 251-258
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Ohlrogge, J.B., and Jaworski, J.G. (1997). Regulation of Fatty Acid Synthesis. Annu Rev Plant Physiol Plant Mol Biol 48, 109-136
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Hlousek-Radojcic, A., Imai, H., and Jaworski, J. (1995). Oleoyl-CoA is not an immediate substrate for fatty acid elongation in developing seeds of Brassica napus. Plant J. 8, 803-809
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Kopka, J., Ohlrogge, J.B., and Jaworski, J.G. (1995). Analysis of in vivo levels of acyl-thioesters with gas chromatography/mass spectrometry of the butylamide derivative. Anal Biochem 224, 51-60
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Ohlrogge, J., Savage, L., Jaworski, J., Voelker, T., and Post-Beittenmiller, D. (1995). Alteration of acyl-acyl carrier protein pools and acetyl-CoA carboxylase expression in Escherichia coli by a plant medium chain acyl-acyl carrier protein thioesterase. Arch Biochem Biophys 317, 185-190
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Slabaugh, M.B., Tai, H., Jaworski, J., and Knapp, S.J. (1995). cDNA clones encoding beta-ketoacyl-acyl carrier protein synthase III from Cuphea wrightii. Plant Physiol 108, 443-444
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Jaworski, J.G., Tai, H., Ohlrogge, J.B., and Post-Beittenmiller, D. (1994). The initial reactions of fatty acid biosynthesis in plants. Prog Lipid Res 33, 47-54
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Tai, H., Post-Beittenmiller, D., and Jaworski, J.G. (1994). Cloning of a cDNA encoding 3-ketoacyl-acyl carrier protein synthase III from Arabidopsis. Plant Physiol 106, 801-802
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Jaworski, J.G., Post-Beittenmiller, D., and Ohlrogge, J.B. (1993). Acetyl-acyl carrier protein is not a major intermediate in fatty acid biosynthesis in spinach. Eur J Biochem 213, 981-987
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Tai, H., and Jaworski, J.G. (1993). 3-Ketoacyl-acyl carrier protein synthase III from spinach (Spinacia oleracea) is not similar to other condensing enzymes of fatty acid synthase. Plant Physiol 103, 1361-1367
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Clough, R.C., Matthis, A.L., Barnum, S.R., and Jaworski, J.G. (1992). Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis. J Biol Chem 267, 20992-20998
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Post-Beittenmiller, D., Jaworski, J.G., and Ohlrogge, J.B. (1991). In vivo pools of free and acylated acyl carrier proteins in spinach. Evidence for sites of regulation of fatty acid biosynthesis. J Biol Chem 266, 1858-1865
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Froehlich, J.E., Poorman, R., Reardon, E., Barnum, S.R., and Jaworski, J.G. (1990). Purification and characterization of acyl carrier protein from two cyanobacteria species. Eur J Biochem 193, 817-825
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